Primary ciliary signaling and function
Institut für Angeborene Immunität, Abteilung Biophysical Imaging
Prof. Dr. rer. nat. Dagmar Wachten
The group Biophysical Imaging applies an interdisciplinary approach combining optogenetics, genetically-encoded biosensors, high-resolution imaging, cell biology, systems biology, and biochemistry to answer a fundamental biological question – what is the physiological function of primary cilia.
Primary cilia are considered as sensory organelles that receive extracellular signals from the environment and transduce this information into a cellular response. Cyclic-nucleotide signaling has been proposed to be an evolutionary conserved signaling pathway, which acts not only in specialized sensory cilia but also in other cilia.
- high-resolution imaging
- in vivo models
- Optogenetics and genetically-encoded biosensors – stimulation and high-throughput read-outs using fluorescence plate reader or FACS
- confocal fluorescence microscopy and spinning-disk microscopy